Annzyme - Angel Special Enzymes Annzyme is a new brand for Angel enzymes products. Focusing on the R&D and promotion of special enzymes and biological products, to tap into the infinite possibilities of enzyme technology in the baking, food taste, brewing, animal feed, pharmaceutical and chemical industries; meanwhile, it gives full play to the advantages of Angel's biological flexible manufacturing to provide client-oriented services for enterprises in the fermentation field. Product Ranges (ผลิตภัณฑ์ที่ให้บริการ) Gluco-amylase GA-150 DESCRIPTION Angel High-Conversion Efficiency Gluco-AmylaseGA-150 is derived through extraction and refinement from theAspergillus niger, with high enzyme activity and glucose conversion. The product is widely used to produce glucose to saccharify liquefied starch from various sources including corn, wheat, barley, rice, tapioca, potato etc. CHARACTERISTICS Name:Gluco-Amylase System name:EC.3.2.1.3,alpha-1,4-D-glycan (glucohydrolase) Appearance: brown liquid Activity: 150,000U/mL (minimum) pH: 3.0 to 5.0 Specific gravity: 1.10 to 1.20 g/ml Effect of pH:the operational range is from pH 3.0 to 5.5; the optimum pH range is from pH 4.0 to 4.4, for maximum enzyme stability, the pH is 4.2. Effect of Temperature:the effective temperature range is from 30~65℃, the optimum temperature range is from 55~60℃, for maximum enzyme stability, the temperature is 60℃. Enzyme loss will dramatically increase if temperature is over 65℃. Commonly, if saccharify liquid is boiled at 80℃, enzyme will be lost completely. MECHANISM The enzyme is an exo-amylase that has the ability to catalyze successive release of glucose units from the non reducing ends of dextrin and oligosaccharide chains by hydrolyzing linear (1.4-α-D), it also slowly catalyzes branched (1.6-α-D) and (1.3-α-D) glucosidic linkages. APPLICATION AND USAGE This product can be used in production of alcohol, starch sugar, monosodium glutamate, antibiotics, citric acid, beer, Chinese rice wine, and other fermentation products. The recommended for optimum industry operation condition is temperature 58~62℃, pH4.0-4.5; commonly adjust pH first, and then add into Gluco-Amylase. The usage of the enzyme comes with the industry, material, technics. We suggest that the concrete usage should be decided by small trials. Normally, the product is added at recommended dose of 0.67~0.77kg/MT DS. 1. Ethanol Industry: Raw materials are braised and cooled to 58~60℃, and adjusted pH4.0-4.5, then the product is added at recommended dose of 0.67~0.77kg /MT, stirred and incubated for 30~60min, then cooled to 30℃for fermentation with yeast. If the continuous saccharification is adopted, the usage of enzyme is added by mash flux, pay attention to prevent the mash to go short circuit without mashing outflow. During the saccharification process, must be constantly stirring. 2. Beer industry: This product can be added before fermentation and saccharification start, which can improve fermentation. Recommended usage is 0.20~0.48kg per ton malt. 3. Brewing industry: In Chinese white, Yellow and koo rice wines industry, it can instead leaven, increase alcohol concentration and shortened fermentation cycles. It can also be applied in vinegar and sauce industry. SPECIFICATION liquid, 150,000U/mL Sealed in Plastic Bucket; Net Weight 30kg/bucket or 1125kg/bucket. STORAGE The product if stored at 25℃below, 12 months shelf life, if under the 4~10℃cold storage, shelf life is 18 months; under warranty, measured activity not less marked activity. Over shelf life, enzymes may decrease, but can still be used, usage should be increased accordingly. The product is bio-active substances, so sunlight, temperature, and humidity can cause inactivation of enzymes. Therefore, should transport and store in cool dry place. Avoid direct exposure to sunlight or moisture. Warehouses should be maintained in a clean, cool, and dry. PRECAUTIONS 1. The product is bio-active substances, inhalation of dust or aerosols may induce sensitization and may cause allergic reactions in sensitized individuals. Unnecessary contact with the product and inhalation of dust should be avoided. 2. In case of contact with the eyes or skin, promptly rinse with the affected area with plenty of water for at least 15 minutes. 3. Starch sources material should contact with the enzyme completely, larger contact area, more time and more benefits, Saccharification mixing fully intermittent, continuous saccharification must flow evenly. To strictly control the temperature 58-60 ℃, heat preservation temperature, short-term high temperature is strictly prohibited. Gluco-amylase GA-260 DESCRIPTION Angel High-Conversion Efficiency Gluco-AmylaseGA-260is derived through extraction and refinementfrom theAspergillus niger, with high enzyme activity and glucose conversion. The product is widely used to produce glucose to saccharify liquefied starch from various sources including corn, wheat, barley, rice, tapioca, potato etc. CHARACTERISTICS Name:Gluco-Amylase System name:EC.3.2.1.3,alpha-1,4-D-glycan(glucohydrolase) Appearance: brown liquid Activity: 260,000U/mL (minimum) pH: 3.0 to 5.0 Specific gravity: 1.10 to 1.20 g/ml Effect of pH:the operational range is from pH 3.0 to 5.5; the optimum pH range is from pH 4.0 to 4.4, for maximum enzyme stability, the pH is 4.2. Effect of Temperature:the effective temperature range is from 30~65℃, the optimum temperature range is from 55~60℃, for maximum enzyme stability, the temperature is 60℃. Enzyme loss will dramatically increase if temperature is over 65℃. Commonly, if saccharify liquid is boiled at 80℃, enzyme will be lost completely. MECHANISM The enzyme is an exo-amylase that has the ability to catalyze successive release of glucose units from the non reducing ends of dextrin and oligosaccharide chains by hydrolyzing linear (1.4-α-D), it also slowly catalyzes branched (1.6-α-D) and (1.3-α-D) glucosidic linkages. APPLICATION AND USAGE This product can be used in production of alcohol, starch sugar, monosodium glutamate, antibiotics, citric acid, beer, Chinese rice wine, and other fermentation products. The recommended for optimum industry operation condition is temperature 58~62℃, pH4.0-4.5; commonly adjust pH first, and then add into Gluco-Amylase. The usage of the enzyme comes with the industry, material, technics. We suggest that the concrete usage should be decided by small trials. Normally, the product is added at recommended dose of 0.45~0.55 kg/MT DS. 1. Ethanol Industry:Raw materials are braised and cooled to 58~60℃, and adjusted pH4.0-4.5, then the product is added at recommended dose of 0.45~0.55 kg /MT, stirred and incubated for 30~60min, then cooled to 30℃for fermentation with yeast. If the continuous saccharification is adopted, the usage of enzyme is added by mash flux, pay attention to prevent the mash to go short circuit without mashing outflow. During the saccharification process, must be constantly stirring. 2. Beer industry:This product can be added before fermentation and saccharification start, which can improve fermentation. Recommended usage is 0.12~0.28 kg per ton malt. 3. Brewing industry:InChinese white, Yellow and koo rice wines industry, it can instead leaven, increase alcohol concentration and shortened fermentation cycles. It can also be applied in vinegar and sauce industry. SPECIFICATION liquid, 260,000U/mL Sealed in Plastic Bucket; Net Weight 30kg/bucket. STORAGE The product if stored at 25℃below, 120d shelf life, if under the 4~10℃cold storage, shelf life is 12 months; under warranty, measured activity not less marked activity. Over shelf life, enzymes may decrease, but can still be used, usage should be increased accordingly. The product is bio-active substances, so sunlight, temperature, and humidity can cause inactivation of enzymes. Therefore, should transport and store in cool dry place. Avoid direct exposure to sunlight or moisture. Warehouses should be maintained in a clean, cool, and dry. PRECAUTIONS The product is bio-active substances, inhalation of dust or aerosols may induce sensitization and may cause allergic reactions in sensitized individuals. Unnecessary contact with the product and inhalation of dust should be avoided. In case of contact with the eyes or skin, promptly rinse with the affected area with plenty of water for at least 15 minutes. Starch sources material should contact with the enzyme completely, larger contact area, more time and more benefits, Saccharification mixing fully intermittent, continuous saccharification must flow evenly. To strictly control the temperature 58-60 ℃, heat preservation temperature, short-term high temperature is strictly prohibited. Thermo-stable alpha-amylase AHA-100T DESCRIPTION Angel Thermo-Stable Alpha-AmylaseAHA-100T is a food-grade starch hydrolyzing α-amylase derived fromBacillus licheniformis.It is an incision enzyme with the characteristics of high temperature, low pH stability and fast viscosity reduction. CHARACTERISTICS Name Thermo-Stable α–Amylase AHA-100T System name EC.3. 2. 1. 1,1,4-α-D-Glucan-glucanohydrolase Activity 100,000U/mL (minimum) Appearance brown liquid pH 4. 8 to 6. 5 Specific gravity 1. 10 to 1. 25 g/ml Effect of pH The enzyme is stable in the range of pH 5. 0 to 8. 0, the optimum pH is in the range of 5. 4 to 5. 8, and it maintains considerable even at pH 5. 0. Effect of Temperature The optimum temperature for this product is 80-100℃, with most effective performance in the range of 105℃to 110℃. The enzyme maintains considerable activity at higher temperatures (115℃or above). APPLICATIONS This product can be widely applied in production of alcohol, beer, Chinese rice wine, vinegar and other fermentation products. The application effective is concerned with raw material type, ratio of material to water, pH, injection/cooking temperature, sustain time and so on. The details of recommended process as below: Corn as raw material Workable Range Optimum Range Ratio of material to water 1:1. 8-1:3 1:2-1:2. 7 pH 4. 8-6. 5 5. 0-6. 0 Precooking(recommended) 80℃ 30 min Injection/cooking temperature 80-100℃ 85-95 ℃ Cooking time 90-120 min Dosage(kg/t dry matter) 0.15-0.40 0.20-0.30 Cassava as raw material Workable Range Optimum Range Ratio of material to water 1:1. 8-1:3 1:2-1:2. 5 pH 4. 8-6. 5 5. 0-6. 0 Injection/cooking temperature 80-100 ℃ 80-95 ℃ Cooking time 90-120 min Dosage (kg/t Dry Matter) 0.12-0.40 0.15-0.25 SPECIFICATION Sealed in Plastic Bucket or IBC Tank; Net Weight: 28kg/bucket or 1000kg/bucket. STORAGE The product if stored at 25℃below, 12months shelf life, if under the 4~10℃cold storage, shelf life is 18 months; PRECAUTIONS 1. The product is bio-active substances, inhalation of dust or aerosols may induce sensitization and may cause allergic reactions in sensitized individuals. Unnecessary contact with the product and inhalation of dust should be avoided. 2. In case of contact with the eyes or skin, promptly rinse with the affected area with plenty of water for at least 15 minutes. Thermo-stable acid alpha-amylase AHA-400 DESCRIPTION Angel Thermo-Stable Acid Alpha-Amylaseis a food-grade starch hydrolyzing α-amylase with high heat and low pH stability derived from Bacillus licheniformis CHARACTERISTICS Name:Thermo-Stable Acid α–Amylase System name:EC.3.2.1.1,1,4-α-D-Glucan-glucanohydrolase Activity: 40,000U/mL (minimum) Appearance: brown liquid pH: 5.5 to 7.0 Specific gravity: 1.10 to 1.25 g/ml Effect of pH The enzyme is stable in the range of pH5.0 to 10.0, and effective in the range of pH4.8 to 7.0, the optimum pH is in the range of 5.5 to 6.5. Effect of Temperature The optimum temperature for this product is above 90℃, with most effective performance in the range of 95℃to 97℃. The enzyme maintains considerable activity at higher temperatures (100℃or above). MECHANISM The enzyme is an endoenzyme that is able to hydrolyze the α-1,4 glycosidic bonds in starch, soluble dextrin, and low-polysaccharides under high temperatures. This enzyme is also able to quickly reduce viscosity of starch slurries to facilitate further handling and processing. APPLICATION AND USAGE This product can be used in production of alcohol, starch sugar, monosodium glutamate, antibiotics, citric acid, beer, Chinese rice wine, and other fermentation products. The recommended for optimum industry operation condition is temperature 90℃~100℃, whereas if steam jet method is used, temperature may increase to 105-110℃,5-7 minutes; pH5.0 ~6.0. 1.Ethanol Industry: After mixing ingredients with water, for each one ton of alcohol add 0.7kg -1kg of enzyme. Adjust the pH to approximately 5.5 only if it is higher than 6.0. Heat and hold the temperature at 95±5℃for 100 minutes. 2.Beer industry:for each ton of ingredient, add 0.2~0.3kg of enzyme. Quickly increase the temperature to 95℃or above. Hold the temperature at 95±5℃for approximately 30 minutes, until liquefying completes. 3.MSG, candy, and glucose:Mix ingredients with water according to specification, adjust the pH, and add 0.3~0.4kg of enzyme to each ton of ingredient. If liquefying is done in a tank, heat the tank to 95℃-100℃and hold the temperature for approximately 30 minutes. Whereas if steam jet method is used, heat steam jet nozzles to 100±5℃and afterwards store liquid in a 95℃environment for 30 minutes or until viscosity meets the requirement. SPECIFICATION liquid, 40,000U/mL Sealed in Plastic Bucket; Net Weight 30kg/bucket or 1000kg/bucket. STORAGE The product if stored at 25℃below, at least 12months shelf life, if under the 4~10℃cold storage, shelf life is 18 months; under warranty, measured activity not less marked activity. Over shelf life, enzymes may decrease, but can still be used, usage should be increased accordingly. The product is bio-active substances, so sunlight, temperature, and humidity can cause inactivation of enzymes. Therefore, should transport and store in cool dry place. Avoid direct exposure to sunlight or moisture. Warehouses should be maintained in a clean, cool, and dry. PRECAUTIONS 1. The product is bio-active substances, inhalation of dust or aerosols may induce sensitization and may cause allergic reactions in sensitized individuals. Unnecessary contact with the product and inhalation of dust should be avoided. 2. In case of contact with the eyes or skin, promptly rinse with the affected area with plenty of water for at least 15 minutes. 3. Starch sources material should contact with the enzyme completely, larger contact area, more time and more benefits. ANGEL α-Cyclodextrin glucanotransferase ANGEL α-Cyclodextrin glucanotransferase is a cyclodextrin glucanotransferase preparation, manufactured by a unique fermentation process using a selected strain belonging to Peanibacillus macerans. CHARACTERISTICS 1. Brown liquid. 2. Optimum Temperature: 40~50℃. 3. Optimum pH: 5. 0~6. 0. MECHANISM ANGEL α-Cyclodextrin glucanotransferase catalyzes the conversion of starch or other α-1,4-glucans to cyclodextrin. APPLICATIONS ANGEL α-Cyclodextrin glucanotransferase is a biocatalyst: catalyzes the conversion of starch to cyclodextrin; has a unique role in VC carbohydrate derivatives、coupling sugar biosynthesis and modification of stevia. USAGE α-Cyclodextrin glucanotransferase can be used for starch liquefaction and conversion of liquefied slurry to produced CDs.We recommend an addition of about 5 units/g of dry starch for liquefaction at 70℃with a 15% starch slurry, then use 5 unitsα-Cyclodextrin glucanotransferase per gram of dry starch at 30℃for 20hr. SPECIFICATIONS 25U/ml (liquid) 25kg/Barrel; 200kg/ Barrel STORAGE The liquid product should be kept between 4℃and 10℃, the declared activity is maintained for at least 3 months. PRECAUTIONS 1. The enzyme preparation is a biological product which contains large amounts of protein. The dust may cause allergy when inhaled. Please take precautions to avoid direct contact with the product. 2. In case of contact with the skin or eyes, rinse thoroughly with copious amount of water. Seek medical advice if lung irritation occurs. Acid protease DESCRIPTION Derived from the fermentation products ofAspergillus niger,Angel acid protease catalyzes amide (peptide) bond hydrolysis in protein or peptide substrates. It is widely used in brewery and manufacture of alcohol, food processing, feed additives, leather processing, etc. CHARACTERISTICS Spec\Type Acid protease Appearance Tawny powder Brown Liquid *Activity Unit (U/g) 50,000~80,000 50,000~100,000 Working Temp 30℃-45℃ Working pH 2.5-5.0 Size or Density ≥80%(Standard Mesh Size 40) --- *Activity: One Activity Unit is the amount of Acid Protease required to liberate the equivalent of 1 μg of Tyrosine per minute under the specified conditions and at 40℃, pH 3.0 APPLICATIONS Acid protease is a water soluble enzyme that is capable of breaking down peptide bonds in proteins. It breaks down and increases protein dispersibility, solubility, palatability and digestibility. The effectiveness of this Acid protease is achieved through continuous technical research and bioengineering refinements. 1. Acid Protease is mainly used in alcohol fermentation. It is ideal for use in the industries that utilize corn, rice, wheat and other high starch content ingredients. With the aid of acid protease, alcohol production becomes more efficient and less time consuming. 2. Acid Protease when used as an additive in high starch products such as bread, or in high protein products such as sausage, helps increase quality by modifying starch content and by enhancing the flavour and taste of the food. In soy sauce production, Acid Protease help increase the concentration of amino acids. In beer production, Acid Protease can even replace the use of Papain and pineapple protease. 3. Acid Protease consumed by the animal along with the feed, will work under the harsh condition of the stomach to hydrolyze protein into peptides and amino acids, promote digestion and absorption, and yield greater return of profit from feed. Enzyme supplements are especially important for young animals because their digestive tracts are not fully developed. 4. Acid Protease is also a very useful tool in the leather and fur industry, as it softens and adds suppleness to lower grade materials, yet at the same time strengthens the root of the fur. Fur treated with Acid Protease will show a noticeable sheen, and further processing such as with dye will allow to more easily bind. Overall, treated leather and fur result in higher quality and provide greater return to its user. USAGE 1. Levels: in alcohol fermentation from corn, the amount of enzyme used usually ranges between 10-16 activity units per gram of ingredient, depending on ingredients and other considerations. An example would be if 14 activity units per gram ingredient is chosen, then for each ton of ingredient, 280 g of 50000 U/g Acid Protease would be used. If 40000 U/g of Acid Protease is used, then 350 g should be needed. In food and feed, the amount used will depend on the specific need of the cllent and should be adjusted accordingly, with a suggestion of 5-10 U/g ingredient to begin with. For soy sauce production, the suggested rate is 25 U/g ingredient, and for beer, 5 U/g ingredient is suggested. 2. Methed: a) Initial fermentation of Alcohol: After filling 1/3 of the ingredients into the fermenting cellar, add in a full amount of livened enzyme. Continue filling cellar and ferment. b) Post-fermentation of alcohol: Add acid protease to the fermenting batch through the successive sugar-adding slot provided on the fermenting cellar. c) Fermenting solids to produce alcohol: After heating the ingredients, cool down to the ideal temperature range and add the whole required amount of acid protease. Be sure the enzyme is thoroughly mixed in. d) Yeast production through fermentation of solid or production of single cell protein feed: After heating, cool the ingredients to 30℃. Add 20 units of Acid Protease per gram of ingredient to lukewarm water, after dissolving, add yeast strain and mix well into batch of ingredients and ferment. SPECIFICATIONS Solid: sealed in plastic inner bag, with a fabric outer bag, net weight 20kg/bag STORAGE Transport and store in cool dry place and avoid direct exposure to sunlight or moisture. Stored below 15℃in original sealed package, activity of solid enzyme remains stable for a period of up to 6 months. PRECAUTIONS The enzyme preparation may irritate the skin and eyes. The dust may cause sensitization when inhaled. Please take precautions to avoid direct contact with the product. Rinsing thoroughly with copious amount of water in case of contacted with the skin or eyes, Seek medical advice if lung irritation occurs. Cellulase DESCRIPTION Cellulase enzyme was derived through fermentation, extraction and refinement fromTrichodermaspecies. It is mainly used to degrade cellulose in fibers to create simple sugars. It is capable of breaking down cellulose and fibers into simple sugar units, resulting in greater utilization of raw ingredients. CHARACTERISTICS Items Cellulase Physical Appearance Light yellow powder *Activity: U/g 10,000 Density (mesh size 40) ≥80% Effective Temperature 40℃-60℃ Effective pH 3. 5-5. 5 Loss on Dry ≤10% *Activity : One activity unit of cellulase is the amount of enzyme, which liberates 1 mg of reducing sugar from carboxymethyl cellulose sodium solution in 1 hour at 50℃ and pH4. 8. MECHANISM Eliminate anti-nutritional effects of soluble xylan in animal feedstuff, decrease chyme viscosity; Break down cell wall of cereal to improve nutrient release and digestibility; Improve animal uniformity and performance; Increase cereal by-product utilization and reduce the cost of feedstuff; Reduce environmental pollution by increasing the absorption of nutrient. APPLICATIONS As an additive to animal feed, cellulase can enhance the digestibility of animal feed, and it is also beneficial to protein digestion and absorption. By adding cellulase into animal feed, animals will show significant signs of increase in weight, milk and egg production, greater ability to adapt to different environments, and a better immune system. When used in food, Chinese medicine, etc., the effectiveness, absorption, and nutritional effects tend to yield better results than those without the enzyme addition. USAGE Recommended dosage is 200g ofmannanase(10,000U/g) per ton of complete feed. The product should be well mixed with feedstuff. SPECIFICATIONS Solid: sealed in plastic inner bag, with a fabric outer bag, net weight 20kg/bag STORAGE Transport and store in cool dry place and avoid direct exposure to sunlight or moisture. Stored below 15℃in original sealed package, activity of solid enzyme remain stable for a period of up to 12 months. PRECAUTIONS The enzyme preparation may irritate the skin and eyes. The dust may cause sensitization when inhaled. Please take precautions to avoid direct contact with the product. Rinsing thoroughly with copious amount of water in case of contacted with the skin or eyes, Seek medical advice if lung irritation occurs. Pectinase DESCRIPTION Angel Pectinaseis derived through extraction and refinementfrom theAspergillus niger. The product is widely used in the manufacture of fruit juice, vegetable juice and wine. CHARACTERISTICS Name:Pectinase Appearance: Yellow powder Activity: 60,000U/g (minimum) Effect of pH:the operational range is from pH 3.0 to 5.0; the optimum pH range is from pH 3.5 to 4.0. Effect of Temperature: the effective temperature range is from 25~65℃, the optimum temperature range is from 40~55℃. MECHANISM The enzyme is one kind of compound bio-catalyst, with multiple ingredients. It mainly contains PMGL, PG, PGL, PE. Pectinase can break the cell wall of fruit and reduce viscosity of juice, speed up the ultra-filtration process, and decrease the processing time strengthen the fruity, reduce the cost, improve the product quality. APPLICATION AND USAGE 1. Fruit and Vegetable Juice: The recommended dosage is 100-400g/t fruit and vegetable slurry, pH 3.5-4.0 at 45~55℃for 1-5 hours. 2. Wine industry: The recommended dosage is 100-300g/t fruit slurry. It can directly add in the fruit slurry or after dilution. SPECIFICATION Powder, 60,000U/g Sealed in cardboardwithplasticinner; Net Weight: 1kg*20/bucket. STORAGE The product if stored at 25℃ below, 12 months shelf life, if under the 4~10℃ cold storage, shelf life is 18 months; under warranty, measured activity not less marked activity. Over shelf life, enzymes may decrease, but can still be used, usage should be increased accordingly. The product is bio-active substances, so sunlight, temperature, and humidity can cause inactivation of enzymes. Therefore, should transport and store in cool dry place. Avoid direct exposure to sunlight or moisture. Warehouses should be maintained in a clean, cool, and dry. PRECAUTIONS 1. The product is bio-active substances, inhalation of dust or aerosols may induce sensitization and may cause allergic reactions in sensitized individuals. Unnecessary contact with the product and inhalation of dust should be avoided. 2. In case of contact with the eyes or skin, promptly rinse with the affected area with plenty of water for at least 15 minutes. Related Blog (บล็อกที่เกี่ยวข้อง) The Role of Acid Protease in Alcohol Fermentation https://en.angelyeast.com/blog/enzymes/the-role-of-acid-protease-in-alcohol-fermentation.html Application of Enzymes on Palatability of Pet Food https://en.angelyeast.com/blog/enzymes/application-of-enzymes-on-palatability-of-pet-food.html Enzyme Enhance Functionality of Plant Protein https://en.angelyeast.com/blog/enzymes/enzyme-enhance-functionality-of-plant-protein.html